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Fakultät für Biologie, Chemie und Geowissenschaften

Abteilung Mykologie: Prof. Dr. Gerhard Rambold

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Peršoh, D; Theuerl, S; Buscot, F; Rambold, G: Towards a universally adaptable method for quantitative extraction of high-purity nucleic acids from soil, Journal of Microbiological Methods, 75, 19–24 (2008), doi:10.1016/j.mimet.2008.04.009 [Link]
Stichworte: DNA, RNA, nucleic acid extraction, soil
Abstract:

[PubMed]

A universally adaptable protocol for quantitative extraction of high-purity nucleic acids from soil is presented. A major problem regarding the extraction of nucleic acids from soil is the presence of humic substances, which interfere with the extraction process itself and in subsequent analytical manipulations. By the approach described here, the humic compounds are precipitated prior to cell lysis with Al2(SO4)3, and thus eliminated prior to the nucleic acid extraction. The protocol allows for removing of a considerable content and range of humic acids and should therefore be applicable for a wide spectrum of soil types. Accordingly, reproducible results in analyses of different soil types are made possible, inclusively for quantitative comparisons.

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